The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go!E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
Technical specifications | |||||||
Additional Info | Can be used for general cloning and blue/white screening | ||||||
Genotype | F’[traD36 lacIq Δ(lacZ) M15 proA+B+] glnV (supE) thi-1 Δ(mcrB-hsdSM)5 (rK- mK- McrB-) thi Δ(lac-proAB) | ||||||
Processing Time | 20 Seconds | ||||||
Product Storage | -70°C to -80°C | ||||||
Transformation Efficiency | 108– 109transformants per µg of plasmid DNA |