The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
Technical specifications | |||||||
Additional Info | Streptomycin Resistant (StrR) and can not be used for Blue-White Selection. | ||||||
Genotype | F- Δ(gpt-proA)62 leuB6 glnV44 (supE44) ara-14 galK2 lacY1 Δ(mcrC-mrr) xyl-5 mtl-1 recA13 thi-1 rpsL20 (SmR) | ||||||
Processing Time | 20 Seconds | ||||||
Product Storage | -70°C to -80°C | ||||||
Transformation Efficiency | 108 – 109 transformants per µg of plasmid DNA |