RR030A/RR006B/RR006A-Ex Taq DNA polymerase, hot-start version- Takara
$181.00 – $1,844.00
Takara Ex Taq HS DNA Polymerase is the hot-start version of our high-performing Takara Ex Taq polymerase (a blend of Takara Taq and a proofreading exonuclease) offering high yield, excellent sensitivity, and fidelity that is 4.5-times higher than Taq polymerase. Antibody-mediated hot-start gives lower background, higher specificity, and allows room temperature reaction assembly. Takara Ex Taq HS DNA polymerase is supplied with 10X buffer (Mg2+) and dNTP mix.
Takara Ex Taq HS DNA polymerase includes a monoclonal antibody to Taq DNA Polymerase that binds to the polymerase and prevents nonspecific amplification due to mispriming and/or formation of primer dimers during reaction assembly. The antibody is then denatured during the initial PCR DNA-denaturation step, releasing the polymerase and allowing DNA synthesis to proceed. A premix Ex Taq is available for fast and sensitive real-time PCR.
Several other formats of Ex Taq are available:
- Individual components ofEx Taq enzyme mix, dNTP mixture, and 10X reaction buffer (with or without Mg2+)—for individual reaction setup and optimization.
- A 2X PCR master mix contains Ex Taq enzyme, reaction buffer (with Mg2+), and dNTP mixture—for a simple PCR setup and minimal pipetting steps.
- A loading dye-added 2X master mix (PerfectShot Ex Taq DNA Polymerase)—for convenient loading of PCR products in agarose gels.
Overview
- Antibody-mediated hot start: lower background, increased specificity, and room-temperature reaction assembly
- High-yield and high-sensitivity PCR
- Excellent on difficult templates
More Information
Applications
- Ideal for high-fidelity, high-yield endpoint PCR
- Amplifications requiring room-temperature reaction assembly
PCR products
PCR products generated with Takara Ex Taq HS contain a mixture of 3′-A overhangs and blunt ends which allows >80% cloning efficiency in T-vectors.
| Cat. # | Product | Size | Description |
| RR030A | Premix Ex Taq™ DNA Polymerase Hot Start Version | 100 Rxns | A hot-start, 2X PCR master mix containing Takara Ex Taq DNA polymerase, optimized buffer, and dNTPs. Please refer to the User Manual for buffer composition. Takara Ex Taq DNA polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq is optimized for amplicons up to 20 kb from genomic DNA, and up to 30 kb from lambda DNA. |
| RR006B | TaKaRa Ex Taq® DNA Polymerase Hot-Start Version | 1,000 Units | A hot-start version of Takara Ex Taq DNA polymerase, which combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq is optimized for amplicons up to 20 kb from genomic DNA, and up to 30 kb from lambda DNA. Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the hot-start polymerase. Cat. # RR006B contains 4 of Cat. # RR006A. Please refer to Cat. # RR006A for complete product documentation and resources. |
| RR006A | TaKaRa Ex Taq® DNA Polymerase Hot-Start Version | 250 Units | A hot-start version of Takara Ex Taq DNA polymerase, which combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq is optimized for amplicons up to 20 kb from genomic DNA, and up to 30 kb from lambda DNA. Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the hot-start polymerase |
| Product | RR030A (Premix Ex Taq™ DNA Polymerase Hot Start Version – 100 Rxns), RR006B (TaKaRa Ex Taq® DNA Polymerase Hot-Start Version – 1,000 Units), RR006A (TaKaRa Ex Taq® DNA Polymerase Hot-Start Version – 250 Units) |
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