RR001C/RR001B/RR001A/RR01CM/RR01BM/RR01AM-Ex Taq DNA polymerase—a robust PCR enzyme with proofreading activity- Takara
$190.00 – $1,844.00
TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR reactions. It can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4.5 times lower, as determined by the Kunkel method. Ex Taq polymerase is supplied with optimized 10X buffer (with or without Mg2+) and dNTPs.
In routine PCR applications, using Ex Taq polymerase and the Ex Taq buffer system results in higher yields of PCR products as compared to standard Taq DNA polymerase.
Several other formats of Ex Taq are available:
- An antibody-mediated hot-start version—for preventing non-specific amplification from mispriming during reaction setup.
- A 2X PCR master mix contains Ex Taq enzyme, reaction buffer (with Mg2+), and dNTPs—for a simple PCR setup and minimal pipetting steps. A loading-dye added versions, PerfectShot Ex Taq DNA Polymerase, is also available for convenient loading of PCR products in agarose gels directly.
Overview
- DNA-proofreading activity allows for high-fidelity and high-sensitivity reactions
- Obtain a wide range of PCR products (<100 bp to 20 kb for genomic DNA targets)
- Optimized 10X Buffer and dNTPs are included—less optimization and more reproducible results than Taq
More Information
Applications
- Ideal for high-fidelity, high-yield endpoint PCR
| Cat. # | Product | Size | Description |
| RR001C | TaKaRa Ex Taq® DNA Polymerase | 3,000 Units | TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the polymerase. Cat. # RR001C contains 12 of Cat. # RR001A. Please refer to Cat. # RR001A for complete product documentation and resources. |
| RR001B | TaKaRa Ex Taq® DNA Polymerase | 1,000 Units | TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the polymerase. Cat. # RR001B contains 4 of Cat. # RR001A. Please refer to Cat. # RR001A for complete product documentation and resources. |
| RR001A | TaKaRa Ex Taq® DNA Polymerase | 250 Units | TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the polymerase. |
| RR01CM | TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) | 3,000 Units | TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ free buffer, MgCl2, and dNTPs are supplied with the polymerase. Cat. # RR01CM contains 12 of Cat. # RR01AM. Please refer to Cat. # RR01AM for complete product documentation and resources. |
| RR01BM | TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) | 1,000 Units | TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ free buffer, MgCl2, and dNTPs are supplied with the polymerase. Cat. # RR01BM contains 4 of Cat. # RR01AM. Please refer to Cat. # RR01AM for complete product documentation and resources. |
| RR01AM | TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) | 250 Units | TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ free buffer, MgCl2, and dNTPs are supplied with the polymerase. |
| Product | RR001C (TaKaRa Ex Taq® DNA Polymerase-3,000 Units), RR001B (TaKaRa Ex Taq® DNA Polymerase-1,000 Units), RR001A (TaKaRa Ex Taq® DNA Polymerase-250 Units), RR01CM (TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer)-3,000 Units), RR01BM (TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer)-1,000 Units), RR01AM (TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer)-250 Units) |
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