The Quick-RNA Midiprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™ column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
Technical specifications | |||||||
Equipment | Microcentrifuge, vortex, vacuum manifold | ||||||
Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. | ||||||
Sample Source | Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™. | ||||||
Size Range | Total RNA ≥ 17 nt | ||||||
Yield | Midiprep: 1 mg RNA (binding capacity), ≥ 200 µl (elution volume) |