Technical Description
pADL™-100 is a phagemid vector designed for high levels of display on the filamentous bacteriophage M13 or equivalent. This vector contains a PelB leader sequence followed by a full-length copy of the gene III sequence and a double-SfiI cloning site to introduce foreign polypeptides on the N-terminal side of the gene III protein. Expression of the fusion is under the tight control of a lac promoter. The presence of a strong RBS, a proteolysis-resistant linker without tags and the absence of amber stop codon all contribute to maximize display on pIII.
Applications
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Phage display at the N-terminal side of gene III protein of the filamentous bacteriophage.
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Polypeptide display.
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Phage display peptide libraries.
Specifications
General Characteristics:
Plasmid Size: 3943
Promoter: lac promoter
Leader Peptide: PelB
Cloning Site: double-BglI/SfiI, NotI-SpeI
Fusion Protein: full length gene III protein
Selection: ampicillin
Replication: oriF1, pMB1
Physical Characteristics:
Concentration: 0.5 µg/µl.
Product Size: 10 µg.
Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile).
Storage Temperature: -20°C.
