DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by many downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
Technical specifications | |||||||
Assay Condition | DNA Degradase in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥ 1 hour. | ||||||
Concentration | 10 U/µl | ||||||
Enzyme Inactivation | 70C for 20 minutes | ||||||
Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C. | ||||||
Unit Definition | One unit (U) is the amount of enzyme required to degrade 1 µg of λ |