The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
