The EZ-96 DNA Methylation-Lightning MagPrep Kit is the fastest magnetic bead-based DNA bisulfite conversion kit for high-throughput and automated methylation analysis. No preparation is necessary with the ready-to-use conversion reagent – simply add this unique reagent to a DNA sample and incubate the reaction for about one hour. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. This magnetic bead-based bisulfite conversion kit produces the highest yields of bisulfite-converted DNA that is ideal for PCR, arrays, library preps, and Next-Generation sequencing.
Technical specifications | |||||||
Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. | ||||||
Conversion | > 99.5% | ||||||
Elution Volume | ≥ 25 µl | ||||||
Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. | ||||||
Input | 100 pg – 2 µg of DNA. | ||||||
Processing Time | 1.5 hours | ||||||
Recovery | > 80% | ||||||
Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |