D4301/D4305- ZymoBIOMICS DNA Microprep Kit- Zymo Research
$223.00 – $302.00
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water | ||||||
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). | ||||||
| Processing Volume | Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into ≥ 10 µl | ||||||
| Product | D4301 (ZymoBIOMICS DNA Microprep Kit (Lysis Tubes) – 50 Preps), D4305 (ZymoBIOMICS DNA Microprep Kit (No Lysis Matrix) – 50 Preps) |
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