The Quick-DNA Viral Kits are viral DNA purification kits that provide for the rapid isolation of high-quality viral DNA from a wide range of biological sources. A uniquely designed buffer is included for the efficient denaturation of viral particles in whole blood (fresh and stored), plasma, serum, tissue, ascites, cultured cells, and from liquid samples. DNA can be eluted with elution buffer or water and is suitable for subsequent PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Technical specifications | |||||||
Applicable For | All sensitive downstream applications such as qPCR and Next-Generation sequencing. | ||||||
Equipment | Centrifuge with microplate carriers | ||||||
Processing Volume | ≤ 200 µl Biological Fluids or ≤ 200 µl cells at a concentration of ≤ 105 cells/ml | ||||||
Purity | Typical A260/A280 ≥ 1.8 | ||||||
Sample Source | Whole blood (fresh and stored), plasma, serum, tissue, ascites, cultured cells, and liquid samples. | ||||||
Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
Size Range | 100 bp – 50 kb | ||||||
Type | Total DNA | ||||||
Yield | Up to 5 µg total DNA can be eluted into ≥ 10 µl |