Technical Description
FAST-Licase™ enables the ultra-fast seamless cloning of two or more DNA fragments. This cloning method is based on the homologous recombination of short, 15 to 20 bp-long overlaps between fragments resulting in precise and unique cloning events. The licase™ complex comprises a series a DNA modifying enzymes that are sequentially activated to achieve an extremely high cloning efficiency in just a few seconds. Competent bacteria with medium transformation efficiency in the range 1 x 108 transformants/µg are usually sufficient to complete cloning projects, thus saving both on time and expenses.
Applications
Assembly of 2 or more DNA fragments.
Cloning of large DNA fragments.
Mutagenesis.
Insertion of short DNA sequences.
Features
DNA assembly between 2 s and 2:30 min (water bath or thermo-cycler).
Very high insertion rate.
Incredible time saver.
High-efficiency competent bacteria unneeded.
No positive control required.
Specifications
Physical characteristics:
Licase™ Complex: 100 µl.
Buffer 5x: 1000 µl.
Storage Temperature: -20°C.
