Rapid amplification of cDNA ends (RACE) is a technique used to obtain the full-length sequences of RNA transcripts. RACE can provide the sequence of an RNA transcript from a short, known sequence within the transcript all the way to the 5′ end (5′ RACE-PCR) or 3′ end (3′ RACE-PCR) of the RNA.

The SMARTer RACE 5’/3′ Kit is an improved version of the SMARTer RACE cDNA Amplification Kit. The SMARTer RACE 5’/3′ Kit provides improved sensitivity, less background and higher specificity over the previous generation of kits. First-strand cDNA generated with this kit can be used directly in 5′- and 3′-RACE PCR reactions. The SMARTer protocol does not involve any of the adaptor ligation steps that other RACE kits incorporate, making the protocol shorter and significantly easier to execute. The new kit is also designed to accommodate larger RNA input volumes (up to 11 µl) and has been shown to perform more efficiently on challenging targets (e.g., those that are long, GC-rich, etc.). The kit includes our highly robust SeqAmp DNA Polymerase for amplification of products, and the In-Fusion HD Cloning Kit for efficient cloning of RACE fragments in the provided linearized vector. The SMARTer RACE 5’/3′ procedure combines SMART first-strand cDNA synthesis technology with a powerful suppression PCR protocol that vastly reduces the background amplification commonly associated with RACE protocols.

• Easy to use—the SMARTer RACE protocol is performed in a single tube, and minimizes handling of both your RNA sample and your synthesized cDNA. Total hands-on time is only four hours.
• Complete kit—the SMARTer RACE 5’/3’ Kit contains all the necessary components (save gene-specific primers) to streamline your process. With this single kit, you can begin first-strand cDNA synthesis and proceed through cloning RACE fragments, recovering successful clones on day two.
• Requires only 10 ng of total RNA—our SMARTer RACE method allows you to utilize small samples, including biopsies, tissue dissections, needle aspirations, and embryonic and rare disease tissues. This optimized protocol significantly reduces non-specific background, which is helpful for analysis of small inputs.
• Specific enrichment for 5′ ends—our carefully designed, chemically treated SMARTer Oligo preferentially hybridizes to the 5′ ends of the cDNA being synthesized. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5′ sequences, thus increasing the likelihood you will amplify the entire sequence of your gene or the upstream regulatory regions.
• No RNA pretreatment required—our SMARTer RACE method requires no RNA pretreatment with DNase. This protocol works with total RNA, as well as with samples that may be contaminated with genomic DNA.