The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Technical specifications | |||||||
Elution Volume | ≥ 25 µl | ||||||
Equipment | Microcentrifuge, vortex, centrifuge w/ microplate carriers | ||||||
Purity | High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 | ||||||
Sample Source | Whole blood, plasma, serum cultured cells, buccal cells, tissue already digested with Proteinase K or mechanically homogenized from human, mouse, rat, etc. are effectively processed using this kit. | ||||||
Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered | ||||||
Workflow | Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. | ||||||
Yield | Up to 5 µg/well total DNA is eluted into ≥30 µl DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg. |